Christie Grace is a consultant in biotechnology specifically on recombinant antibodies, CRISPR, mRNA, gene synthesis & Quality control. On this episode we discuss Lipid Nanoparticles (LNP) - and how they relate to mRNA technology as the delivery mechanism. We dive into the known adverse outcomes related to the LNP's with regards to clotting, damage to endothelial cells, fertility and more as well as how this was known to the CDC and NIH for years prior. The freeze-thaw process is also out to be questioned due to known ways this degrades the...
Christie Grace is a consultant in biotechnology specifically on recombinant antibodies, CRISPR, mRNA, gene synthesis & Quality control. On this episode we discuss Lipid Nanoparticles (LNP) - and how they relate to mRNA technology as the delivery mechanism. We dive into the known adverse outcomes related to the LNP's with regards to clotting, damage to endothelial cells, fertility and more as well as how this was known to the CDC and NIH for years prior. The freeze-thaw process is also out to be questioned due to known ways this degrades the LNP's.
0:45 Intro
04:30 What is it about the mRNA tech that's so different? What about in the lab?
10:30 Why is the timeline questionable for the trials?
12:00 What is mRNA
23:45 What & Why are LNP's important (lipid nano particles)
41:00 mRNA fragmentation within batches/vials
47:15 Could fragmentation be an accident
53:00 Freeze thaw
54:30 Ribosomal overload
1:00:00 Real life freeze thaw issues (data at the 53 min mark)
1:09:00 Does the initial trial pass the sniff test?
1:18:30 What does this mean for the public?
1:29:00 Where does this all end?
1:46:50 Top tips to stay aware
1:54:40 Where to find Christie
Follow Christie
https://twitter.com/_HeartofGrace_
Substack
https://christielauragrace.substack.com/p/lnp-liquid-nano-phantasmagoria
Follow me
Twitter - Https://twitter.com/sunnypodcasts
Insta - https://instagram.com/sunnys91
What are Ionizable Lipids (for use in Lipic Nano Particles)
https://broadpharm.com/blog/what-are-ionizable-lipids#:~:text=Ionizable%20lipids%20are%20a%20class,be%20protonated%20at%20low%20pH
Degredation of LNP
https://www.cell.com/biophysj/fulltext/S0006-3495(21)00241-1?s=03
Safety data sheets
https://cdn.caymanchem.com/cdn/msds/15100m.pdf
https://avantilipids.com/assets/products/attachments/850365C_E_7950_US.pdf
LNP Oxidative stress & toxicity (clots, cancer inducing etc.)
https://stacks.cdc.gov/view/cdc/21130
LNP Reproduction
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294055/
Misfolded proteins
https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.11-186197
Aussie TGA specific batches and allocations
https://www.tga.gov.au/batch-release-assessment-covid-19-vaccines
Japan foreign substances
https://asia.nikkei.com/Spotlight/Coronavirus/COVID-vaccines/1.6m-Moderna-doses-withdrawn-in-Japan-over-contamination
Freeze thaw
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2794342/
https://threadreaderapp.com/thread/1603149749942624263.html
Spike found in brain & heart tissue
https://www.mdpi.com/2076-393X/10/10/1651
"Dansen met Janssen" (You'll have to translate the page)
https://www.metronieuws.nl/in-het-nieuws/binnenland/2021/09/kapotte-koeling-janssen-vaccins-dansen-met-janssen/
What's spike
https://www.uniprot.org/uniprotkb/P0DTC2/entry
Safety of LNP mRNA rat/monkey
https://pubmed.ncbi.nlm.nih.gov/29191134/
Circulating spike protein
https://www.ahajournals.org/doi/epdf/10.1161/CIRCULATIONAHA.122.061025
Coagulation triggered by positively charged surfaces
https://www.duo.uio.no/bitstream/handle/10852/61833/Sperling%2Bet%2Bal.pdf?sequence=1&isAllowed=y
Phosphatidylethanolamine & coagulation with VLDL
https://www.ahajournals.org/doi/full/10.1161/atvb.21.10.1695
Mouse models and human lipoprotein suitability
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4712022/
Naturally occurring cholesterol analogues in LNP
https://www.nature.com/articles/s41467-020-14527-2
Mechanisms for DVT & Management
https://cdt.amegroups.com/article/view/16872/18087#:~:text=Virchow's%20Triad%2C%20first%20described%20in,cause%20thrombus%20formation%20(8)
Plasma lipids & Clotting
https://onlinelibrary.wiley.com/doi/full/10.1002/rth2.12017
Phosphatidylethanolamine
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778737/
https://www.ahajournals.org/doi/full/10.1161/atvb.21.10.1695
Clotting and lipids
https://onlinelibrary.wiley.com/doi/full/10.1002/rth2.12017
