Gene Protein Assay(GPA): Unveiling Tumor Heterogeneity in HER2/neu Positive Breast Cancer
Authors: Dr. Tanuja Shet, Dr. Aditi Rathi
Introduction: HER2/neu gene amplification on Fluorescence in-situ hybridization (FISH) is defined as average HER2neu gene signals > 4 and HER2:CEP17 ratio of > 2, in breast cancer. However, with tumor heterogeneity interpretation of these cut-offs is challenging and some cases test as borderline. We attempted a study analyzing Gene protein assay (GPA) which combines HER2/neu immunohistochemistry (IHC), and D-DISH (dual color dual in-situ hybridization) assay to help resolve this issue.
Methods: A total of 31 cases reported as HER2/neu amplified with intermixed tumor heterogeneity on FISH were taken for this study. The original HER2/neu count was between 4 to 6 and ratio near 2 in most cases. GPA was done using 4B5 HER2/neu IHC and Roche/Ventana D-DISH kit on Ventana Ultra machine by doing IHC first followed by D-DISH.
Result: On GPA, 3/31 cases were reported as non-amplified, and the rest were amplified. Advantage of GPA was that it helped count D-DISH as per membrane staining and identified more tumor heterogeneity in contrast to FISH in six cases. The results of the remaining cases were the same.
HistoHelp: The Truth Behind Troubleshooting
HistoHelp: Equipment Maintenance Tips
HistoHelp: Education Can Move You Into Management
HistoHelp: Artificial Intelligence - Not Scary Afterall!
2019 Poster Podcast Interviews: P16- A New and Innovative Antibody p16 INK4a [BC42]: An IHC Comparative Analysis with Clone [E6H4]
2019 Poster Podcast Interviews: P23-Developing an Immunohistochemistry (IHC) assay targeting PTK7, a novel target with a novel assay
2019 Poster Podcast Interviews: P01-Epithelioid haemangioendothelioma (EHE) of pleura
2019 Poster Podcast Interviews: P44-Conventional versus Tissue-Tek Xpress x120 Rapid Tissue Processing: A blind comparison study using large surgical tissues.
NSH Advocacy: Recognition Under CLIA
2019 Poster Podcast Interviews: P33-Morphologic Measurement Of Tuberculoid Lung Disease By Multiple Methods
2019 Poster Podcast Interviews: P48-In Situ Imaging of Tissue Remodeling with Collagen Hybridizing Peptides
2019 Poster Podcast Interviews: P30-The effect of different decalcifying reagents on IHC and ISH assays
2019 Poster Podcast Interviews: P26-Digital Image analysis in GSK (- a useful tool for safety, efficacy and animal modelling)
2019 Poster Podcast Interviews: P22-The Antibody Crisis: Leveraging machine learning for AI-assisted antibody selection
2019 Poster Podcast Interviews: P52-7 Plex Whole Slide Serial Section Alignment of UltiMapper PD-L1 and PD-1 I/O Multiplex Kits with True Reference H&E
2019 Poster Podcast Interviews: P40- Flex-Array A Novel Multi-Well Vessel System for the Immobilization and Multi-Modal Testing of Intact Formalin-Fixed Paraffin-Embedded (FFPE) Cells or Tissues
HistoHelp: Distruptive Technology
HistoHelp: Don't Forget the Basics
HistoHelp: Working in Research vs. Clinical
HistoHelp: Cutting Thin Paraffin Sections
Create your
podcast in
minutes
It is Free
DNA Today: A Genetics Podcast
Museum of the Missing
Strange by Nature Podcast
Sasquatch Chronicles
Hidden Brain