In this episode of the Epigenetics Podcast, we caught up with Karolin Luger, Ph.D., from the University of Colorado in Boulder to talk about her work on solving the crystal structure of the nucleosome and on how histone chaperones like FACT act on chromatin.
During her postdoc with Timothy Richmond at the Swiss Federal Institute of Technology in Zürich, Karolin Luger was the first author on an all-time classic paper called "Crystal structure of the nucleosome core particle at 2.8 A resolution" which was published in Nature. This article was published more than 20 years ago now and it has been cited about 9000 times.
After completing her postdoc, she moved to Colorado to set up her own lab where she continued to work on the structure of the nucleosome and the factors that influence their structure. The most recent Nature paper published by her lab investigated how the FACT complex promotes both disassembly and reassembly of nucleosomes during gene transcription, DNA replication, and DNA repair.
In this interview, we discuss the efforts that went into solving the crystal structure of the nucleosome back in 1997, her work on histone chaperones, and her recent work on how FACT keeps nucleosomes intact after gene transcription.
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Enhancer Communities in Adipocyte Differentiation (Susanne Mandrup)
Transposable Elements in Gene Regulation and Evolution (Marco Trizzino)
Hydroxymethylation Landscape in Immunecells (Marcela Sjöberg)
Single Cell Epigenomics in Neuronal Development (Tim Petros)
Oncohistones as Drivers of Pediatric Brain Tumors (Nada Jabado)
Characterization of Epigenetic States in the Oligodendrocyte Lineage (Gonçalo Castelo-Branco)
Multiple challenges of ATAC-Seq, Points to Consider (Yuan Xue)
The Effect of lncRNAs on Chromatin and Gene Regulation (John Rinn)
Epigenetic Clocks and Biomarkers of Ageing (Morgan Levine)
Epigenetic and Metabolic Regulation of Early Development (Jan Żylicz)
Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)
The Role of Histone Dopaminylation and Serotinylation in Neuronal Plasticity (Ian Maze)
The Role of Blimp-1 in Immune-Cell Differentiation (Erna Magnúsdóttir)
Multimodal Characterization of Cellular Identity | CITE-Seq and Beyond (Peter Smibert, VP of 10X Genomics)
The Effect of Mechanotransduction on Chromatin Structure and Transcription in Stem Cells (Sara Wickström)
Single-Cell Technologies using Microfluidics (Ben Hindson, CSO of 10x Genomics)
Epigenome-based Precision Medicine (Eleni Tomazou)
Epigenetics and Epitranscriptomics in Cancer (Manel Esteller)
Enhancer-Promoter Interactions During Development (Yad Ghavi-Helm)
The Effect of Vitamin D on the Epigenome (Folami Ideraabdullah)
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