Podbean logo
  • Discover
  • Podcast Features

    Your all-in-one podcasting solution.

    Podcast Studio

    Easy-to-use audio recorder app.

  • Livestream

    High-performing audio live, without limits.

  • Podcast App

    The best podcast player & podcast app.

  • Ads Marketplace

    Join Ads Marketplace to earn money
    through sponsorship on your podcast.

    PodAds

    Manage your ads with dynamic ad insertion capability.

  • Patron & Paid Content

    The seamless way for fans to support you directly
    from your podcast.

  •  
  • All Arts Business Comedy Education
  • Fiction Government Health & Fitness History Kids & Family
  • Leisure Music News Religion & Spirituality Science
  • Society & Culture Sports Technology True Crime TV & Film
  • Live
  • Log in
  • Start your podcast for free
  • Podcasting
    • Podcast Features
    • Live Stream
    • PodAds
    • Podcast App
    • Podcast Studio
  • Monetization
    • Premium
    • Patron
    • Ads Marketplace
  • Enterprise
  • Pricing
  • Discover
  • Log in
    Sign up free
Epigenetics Podcast

Epigenetics Podcast

Science

Multimodal Characterization of Cellular Identity | CITE-Seq and Beyond (Peter Smibert, VP of 10X Genomics)

Multimodal Characterization of Cellular Identity | CITE-Seq and Beyond (Peter Smibert, VP of 10X Genomics)

2022-04-14
Download

In this episode of the Epigenetics Podcast, we speak with Peter Smibert, Vice President of Biology at 10X Genomics to talk about an exciting new method in Multimodal Characterization of Cellular Identity using Barcoding.

During his time at the New York Genome Center, Peter Smibert was instrumental in the development of a new method called "Cellular Indexing of Transcriptomes and Epitopes by Sequencing" short CITE-Seq. This method enables the characterization of a cell's transcriptome, while at the same time, also allows the characterization of the cell's protein surface markers - at the single cell level. In CITE-Seq, sequencing adapters are coupled to antibodies that recognize surface proteins, which can then be detected by sequencing.

Further advancements of the CITE-Seq method led to the launch of BioLegend’s TOTAL-Seq and the integration of scATAC-Seq into the workflow. With the integration of scATAC-Seq in the CITE-Seq protocol, it is now possible to characterize single-cells along the path of the central dogma of biology, this is why the method called DOGMA-Seq.

 

References

  • https://cite-seq.com

  • Baron, M., Yanai, I. New skin for the old RNA-Seq ceremony: the age of single-cell multi-omics. Genome Biol 18, 159 (2017). https://doi.org/10.1186/s13059-017-1300-5

  • Stoeckius, M., Zheng, S., Houck-Loomis, B. et al. Cell Hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomics. Genome Biol 19, 224 (2018). https://doi.org/10.1186/s13059-018-1603-1

  • Stoeckius, M., Hafemeister, C., Stephenson, W. et al. Simultaneous epitope and transcriptome measurement in single cells. Nat Methods 14, 865–868 (2017). https://doi.org/10.1038/nmeth.4380

  • Mimitou, E.P., Cheng, A., Montalbano, A. et al. Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells. Nat Methods 16, 409–412 (2019). https://doi.org/10.1038/s41592-019-0392-0

     

Related Episodes

  • Epigenome-based Precision Medicine (Eleni Tomazou)

  • ATAC-Seq, scATAC-Seq and Chromatin Dynamics in Single-Cells

  • Investigating the Dynamics of Epigenetic Plasticity in Cancer with Single Cell Technologies (Céline Vallot)

 

Contact

  • Active Motif on Twitter

  • Epigenetics Podcast on Twitter

  • Active Motif on LinkedIn

  • Active Motif on Facebook

  • Email: podcast@activemotif.com

view more

More Episodes

Effects of Environmental Cues on the Epigenome and Longevity (Paul Shiels)
2023-03-23
The Epigenetics of Human Sperm Cells (Sarah Kimmins)
2023-03-09
Transgenerational Inheritance and Evolution of Epimutations (Peter Sarkies)
2023-02-23
Transcription Elongation Control by the Paf1 Complex (Karen Arndt)
2023-02-09
Molecular Mechanisms of Chromatin Modifying Enzymes (Karim-Jean Armache)
2023-01-26
The Role of PHF13 in Chromatin and Transcription (Sarah Kinkley)
2023-01-12
Genome Organization Mediated by RNA Polymerase II (Argyrys Papantonis)
2022-12-15
The Role of Pioneer Factors Zelda and Grainyhead at the Maternal-to-Zygotic Transition (Melissa Harrison)
2022-12-01
Epigenetics in Human Malaria Parasites (Elena Gómez-Diaz)
2022-11-17
Bioinformatic Analysis in Epigenetics Research (Nick Pervolarakis)
2022-11-03
Anchor-Based Bisulfite Sequencing (Ben Delatte)
2022-10-20
Enhancer Communities in Adipocyte Differentiation (Susanne Mandrup)
2022-10-06
Transposable Elements in Gene Regulation and Evolution (Marco Trizzino)
2022-09-22
Hydroxymethylation Landscape in Immunecells (Marcela Sjöberg)
2022-09-08
Single Cell Epigenomics in Neuronal Development (Tim Petros)
2022-08-25
Oncohistones as Drivers of Pediatric Brain Tumors (Nada Jabado)
2022-08-11
Characterization of Epigenetic States in the Oligodendrocyte Lineage (Gonçalo Castelo-Branco)
2022-07-28
Multiple challenges of ATAC-Seq, Points to Consider (Yuan Xue)
2022-07-14
The Effect of lncRNAs on Chromatin and Gene Regulation (John Rinn)
2022-06-30
Epigenetic Clocks and Biomarkers of Ageing (Morgan Levine)
2022-06-23
  • ←
  • 1
  • 2
  • 3
  • 4
  • 5
  • →
012345678910111213141516171819

Get this podcast on your
phone, FREE

Download Podbean app on App Store Download Podbean app on Google Play

Create your
podcast in
minutes

  • Full-featured podcast site
  • Unlimited storage and bandwidth
  • Comprehensive podcast stats
  • Integrate with iTunes and Google
    store
  • Make money with your podcast
Get started

It is Free

  • Podcast Services

    • Podcast Features
    • Pricing
    • Enterprise Solution
    • Private Podcast
    • The Podcast App
    • Live Stream
    • Audio Recorder
    • Remote Recording
  •  
    • Create a Podcast
    • Video Podcast
    • Start Podcasting
    • Start Radio Talk Show
    • Education Podcast
    • Church Podcast
    • Nonprofit Podcast
    • Get Sermons Online
    • Free Audiobooks
  • MONETIZATION & MORE

    • Podcast Advertising
    • Dynamic Ads Insertion
    • Patron Program
    • Affiliate Program
    • Switch to Podbean
    • Submit Your Podcast
    • Podbean Plugins
    • Developers
    • Badges
  • KNOWLEDGE BASE

    • How to Start a Podcast
    • How to Start a Live Podcast
    • How to Monetize a podcast
    • How to Promote Your Podcast
    • How to Use Group Recording
  • Support

    • Support Center
    • Podbean Blog
    • What’s New
    • Free Webinars
    • Podcast Events
    • Podbean Academy
    • Podcasting Smarter
    • Resources
  • Podbean

    • About Us
    • Careers
    • Press and Media
    • Terms of Use
    • Privacy Policy
    • Contact Us

Copyright © 2006-2023 Podbean.com