Optical Projection Tomography for Infarct Assessment

Is optical projection tomography (OPT) useful for producing more than just beautiful images? Yes, according to Zhao et al, who employed the innovative OPT methodology to quantify infarct size experimentally. In this podcast, Associate Editor Ajay Shah (King's College London) interviews lead author Gillian Gray (University of Edinburgh) and content expert Michael Marber (The Rayne Institute, St. Thomas' Hospital, King’s College London) about how Gray and colleagues used OPT to image the heart and capture 3-dimensional measurement of infarct volume. How does OPT stack up against standard histology and MRI? What exactly is giving rise to the autofluorescence signal when using OPT? Is there a future for OPT as a method of measuring infarct volume to transcend biological variations and the limitations of measuring individual sections of infarcted tissue? Listen to find out.

Xiaofeng Zhao, Junxi Wu, Calum D. Gray, Kieran McGregor, Adriano G Rossi, Harris Morrison, Maurits A Jansen, Gillian A Gray Optical projection tomography permits efficient assessment of infarct volume in the murine heart post-myocardial infarction Am J Physiol Heart Circ Physiol, published online June 12, 2015, DOI: 10.1152/ajpheart.00233.2015.