In this episode of the Epigenetics Podcast, we caught up with Nick Pervolarakis from Active Motif to talk about bioinformatic analysis in epigenetics research.
While many “bench scientists” are familiar with the workflows of ChIP-Seq, ATAC-Seq and CUT&Tag, and even the preparation and analysis of the libraries, the steps between sequencing and fully analyzed data is sometimes thought of as a mystery known only to bioinformatic experts. Most of us have some understanding that the raw data is usually in a file format called a FASTQ. But how do we get from FASTQ files to peaks on a genome browser? This Podcast Episode will provide a peek behind the curtain of the informatic analysis we perform at Active Motif, as part of our end-to-end epigenetic services.
References
Life in the FASTQ Lane
Bioinformatics Resource Center
Epigenetic Services
Related Episodes
Multiple challenges of ATAC-Seq, Points to Consider (Yuan Xue)
Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)
Multiple Challenges in ChIP (Adam Blattler)
Contact
Active Motif on Twitter
Epigenetics Podcast on Twitter
Active Motif on LinkedIn
Active Motif on Facebook
Email: podcast@activemotif.com
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Transgenerational Inheritance and Epigenetic Imprinting in Plants (Mary Gehring)
When is a Peak a Peak? (Claudio Cantù)
Analysis of 3D Chromatin Structure Using Super-Resolution Imaging (Alistair Boettiger)
Gene Dosage Alterations in Evolution and Ageing (Claudia Keller Valsecchi)
Structural Analysis of Nucleosomes During Transcription (Lucas Farnung)
DNA Methylation Alterations in Neurodegenerative Diseases (Paula Desplats)
scDamID, EpiDamID and Lamina Associated Domains (Jop Kind)
Circulating Epigenetic Biomarkers in Cancer (Charlotte Proudhon)
Epigenetic Landscapes During Cancer (Luciano Di Croce)
Formation of CenH3-deficient Kinetochores (Ines Drinnenberg)
Effects of Environmental Cues on the Epigenome and Longevity (Paul Shiels)
The Epigenetics of Human Sperm Cells (Sarah Kimmins)
Transgenerational Inheritance and Evolution of Epimutations (Peter Sarkies)
Transcription Elongation Control by the Paf1 Complex (Karen Arndt)
Molecular Mechanisms of Chromatin Modifying Enzymes (Karim-Jean Armache)
The Role of PHF13 in Chromatin and Transcription (Sarah Kinkley)
Genome Organization Mediated by RNA Polymerase II (Argyrys Papantonis)
The Role of Pioneer Factors Zelda and Grainyhead at the Maternal-to-Zygotic Transition (Melissa Harrison)
Epigenetics in Human Malaria Parasites (Elena Gómez-Diaz)
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