In this episode of the Epigenetics Podcast, we caught up with Nick Pervolarakis from Active Motif to talk about bioinformatic analysis in epigenetics research.
While many “bench scientists” are familiar with the workflows of ChIP-Seq, ATAC-Seq and CUT&Tag, and even the preparation and analysis of the libraries, the steps between sequencing and fully analyzed data is sometimes thought of as a mystery known only to bioinformatic experts. Most of us have some understanding that the raw data is usually in a file format called a FASTQ. But how do we get from FASTQ files to peaks on a genome browser? This Podcast Episode will provide a peek behind the curtain of the informatic analysis we perform at Active Motif, as part of our end-to-end epigenetic services.
References
Life in the FASTQ Lane
Bioinformatics Resource Center
Epigenetic Services
Related Episodes
Multiple challenges of ATAC-Seq, Points to Consider (Yuan Xue)
Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)
Multiple Challenges in ChIP (Adam Blattler)
Contact
Active Motif on Twitter
Epigenetics Podcast on Twitter
Active Motif on LinkedIn
Active Motif on Facebook
Email: podcast@activemotif.com
c8dqseb1h2Zyf2blC15qkyPgu9sB5stin27nqhzP
Anchor-Based Bisulfite Sequencing (Ben Delatte)
Enhancer Communities in Adipocyte Differentiation (Susanne Mandrup)
Transposable Elements in Gene Regulation and Evolution (Marco Trizzino)
Hydroxymethylation Landscape in Immunecells (Marcela Sjöberg)
Single Cell Epigenomics in Neuronal Development (Tim Petros)
Oncohistones as Drivers of Pediatric Brain Tumors (Nada Jabado)
Characterization of Epigenetic States in the Oligodendrocyte Lineage (Gonçalo Castelo-Branco)
Multiple challenges of ATAC-Seq, Points to Consider (Yuan Xue)
The Effect of lncRNAs on Chromatin and Gene Regulation (John Rinn)
Epigenetic Clocks and Biomarkers of Ageing (Morgan Levine)
Epigenetic and Metabolic Regulation of Early Development (Jan Żylicz)
Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)
The Role of Histone Dopaminylation and Serotinylation in Neuronal Plasticity (Ian Maze)
The Role of Blimp-1 in Immune-Cell Differentiation (Erna Magnúsdóttir)
Multimodal Characterization of Cellular Identity | CITE-Seq and Beyond (Peter Smibert, VP of 10X Genomics)
The Effect of Mechanotransduction on Chromatin Structure and Transcription in Stem Cells (Sara Wickström)
Single-Cell Technologies using Microfluidics (Ben Hindson, CSO of 10x Genomics)
Epigenome-based Precision Medicine (Eleni Tomazou)
Epigenetics and Epitranscriptomics in Cancer (Manel Esteller)
Enhancer-Promoter Interactions During Development (Yad Ghavi-Helm)
Create your
podcast in
minutes
It is Free
DNA Today: A Genetics Podcast
Museum of the Missing
Strange by Nature Podcast
Sasquatch Chronicles
Hidden Brain