Chromatin Immunoprecipitation (ChIP) was first developed in Drosophila, where interactions of RNA Pol II with genes were investigated (Mol. Cell. Biol. August 1985 vol. 5 no. 8 2009-2018). Then, in a 1993 publication, (Genes & Dev. 1993 7:592-604), the group of James Broach described the association of histone acetylation state with transcriptional gene silencing in yeast. The technique was first used successfully in mammalian cells by Richard Treisman's group, published in 1998 (Cell (1998) 92:475-87).
Chromatin Immunoprecipitation is used to link specific states of chromatin to individual loci in a cell, to understand how genes are regulated, and to decipher the Histone Code. In this Episode, we discuss the multiple challenges of ChIP experiments and the difficulties that can arise during different steps of the process.
Epigenetics News:
Multiple Challenges in ChIP
Anchor-Based Bisulfite Sequencing (Ben Delatte)
Enhancer Communities in Adipocyte Differentiation (Susanne Mandrup)
Transposable Elements in Gene Regulation and Evolution (Marco Trizzino)
Hydroxymethylation Landscape in Immunecells (Marcela Sjöberg)
Single Cell Epigenomics in Neuronal Development (Tim Petros)
Oncohistones as Drivers of Pediatric Brain Tumors (Nada Jabado)
Characterization of Epigenetic States in the Oligodendrocyte Lineage (Gonçalo Castelo-Branco)
Multiple challenges of ATAC-Seq, Points to Consider (Yuan Xue)
The Effect of lncRNAs on Chromatin and Gene Regulation (John Rinn)
Epigenetic Clocks and Biomarkers of Ageing (Morgan Levine)
Epigenetic and Metabolic Regulation of Early Development (Jan Żylicz)
Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)
The Role of Histone Dopaminylation and Serotinylation in Neuronal Plasticity (Ian Maze)
The Role of Blimp-1 in Immune-Cell Differentiation (Erna Magnúsdóttir)
Multimodal Characterization of Cellular Identity | CITE-Seq and Beyond (Peter Smibert, VP of 10X Genomics)
The Effect of Mechanotransduction on Chromatin Structure and Transcription in Stem Cells (Sara Wickström)
Single-Cell Technologies using Microfluidics (Ben Hindson, CSO of 10x Genomics)
Epigenome-based Precision Medicine (Eleni Tomazou)
Epigenetics and Epitranscriptomics in Cancer (Manel Esteller)
Enhancer-Promoter Interactions During Development (Yad Ghavi-Helm)
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