Chromatin Immunoprecipitation (ChIP) was first developed in Drosophila, where interactions of RNA Pol II with genes were investigated (Mol. Cell. Biol. August 1985 vol. 5 no. 8 2009-2018). Then, in a 1993 publication, (Genes & Dev. 1993 7:592-604), the group of James Broach described the association of histone acetylation state with transcriptional gene silencing in yeast. The technique was first used successfully in mammalian cells by Richard Treisman's group, published in 1998 (Cell (1998) 92:475-87).
Chromatin Immunoprecipitation is used to link specific states of chromatin to individual loci in a cell, to understand how genes are regulated, and to decipher the Histone Code. In this Episode, we discuss the multiple challenges of ChIP experiments and the difficulties that can arise during different steps of the process.
Epigenetics News:
Multiple Challenges in ChIP
Chromatin Profiling: From ChIP to CUT&RUN, CUT&Tag and CUTAC (Steven Henikoff)
Effects of DNA Methylation on Diabetes (Charlotte Ling)
Nutriepigenetics: The Effects of Diet on Behavior (Monica Dus)
Investigating the Dynamics of Epigenetic Plasticity in Cancer with Single Cell Technologies (Céline Vallot)
Epigenetic Regulation of Stem Cell Self-Renewal and Differentiation (Margaret “Peggy” Goodell)
Genome-Wide Investigation of Epigenetic Marks and Nucleosome Positioning (Keji Zhao)
The Role of lncRNAs in Tumor Growth and Treatment (Sarah Diermeier)
Unraveling Mechanisms of Chromosome Formation (Job Dekker)
Transcription and Polycomb in Inheritance and Disease (Danny Reinberg)
The Epigenetics of COVID-19
Epigenetic Reprogramming During Mammalian Development (Wolf Reik)
In vivo Nucleosome Structure and Dynamics (Srinivas Ramachandran)
Pioneer Transcription Factors and Their Influence on Chromatin Structure (Ken Zaret)
The Role of Small RNAs in Transgenerational Inheritance in C. elegans (Oded Rechavi)
Development of Site-Specific ChIP Technologies (Hodaka Fujii)
Regulation of Chromatin Organization by Histone Chaperones (Geneviève Almouzni)
How the "Fragile Nucleosome" Science Community Came to Life (Christine Cucinotta, Melvin Noe Gonzalez)
Epigenetic Influence on Memory Formation and Inheritance (Isabelle Mansuy)
Influence of Dynamic RNA Methylation on Gene Expression (Chuan He)
How to Publish in Nature: Lessons from the ENCODE Consortium (Michelle Trenkmann, Senior Editor at Nature)
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