Chromatin Immunoprecipitation (ChIP) was first developed in Drosophila, where interactions of RNA Pol II with genes were investigated (Mol. Cell. Biol. August 1985 vol. 5 no. 8 2009-2018). Then, in a 1993 publication, (Genes & Dev. 1993 7:592-604), the group of James Broach described the association of histone acetylation state with transcriptional gene silencing in yeast. The technique was first used successfully in mammalian cells by Richard Treisman's group, published in 1998 (Cell (1998) 92:475-87).
Chromatin Immunoprecipitation is used to link specific states of chromatin to individual loci in a cell, to understand how genes are regulated, and to decipher the Histone Code. In this Episode, we discuss the multiple challenges of ChIP experiments and the difficulties that can arise during different steps of the process.
Epigenetics News:
Multiple Challenges in ChIP
The Role of Non-Histone Proteins in Chromosome Structure and Function During Mitosis (Bill Earnshaw)
Effects of DNA Methylation on Chromatin Structure and Transcription (Dirk Schübeler)
CpG Islands, DNA Methylation, and Disease (Adrian Bird)
Biophysical Modeling of 3-D Genome Organization (Leonid Mirny)
From Nucleosome Structure to Function (Karolin Luger)
Identification of Functional Elements in the Genome (Bing Ren)
Hi-C and Three-Dimensional Genome Sequencing (Erez Lieberman Aiden)
Chromatin Structure and Dynamics at Ribosomal RNA Genes (Tom Moss)
Epigenetic Origins Of Heterogeneity And Disease (Andrew Pospisilik)
PIXUL: On the Leading Edge of Chromatin Shearing (Karol Bomsztyk and Tom Matula)
Influence of Histone Variants on Chromatin Structure and Metabolism (Markus Buschbeck)
Epigenetic Mechanisms of Aging and Longevity (Shelley Berger)
Epigenetics & Glioblastoma: New Approaches to Treat Brain Cancer (Lucy Stead)
The Past, Present, and Future of Epigenetics (Joe Fernandez, founder of Active Motif)
The Interchromatin Network Model (Ana Pombo)
Dosage Compensation in Drosophila (Asifa Akhtar)
Spatial Organization of the Human Genome (Wendy Bickmore)
Heterochromatin and Phase Separation (Gary Karpen)
Diabetes and Epigenetics (Jean-Sébastien Annicotte)
Epigenetics and X-inactivation (Edith Heard)
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