The Nucleosome is the basic building unit of chromatin. It consists out of 147 base pairs of double stranded DNA wrapped around the Histone core octamer that consists out of 2 copies of each dimer of H2A/H2B, and H3/H4. Nucleosomes are organized like "beads on a string" to form a modifiable regulatory basis for higher order structures of chromatin. The first images of the nucleosome as a particle was published by our guests Ada and Don Olins from the University of New England in 1974 (Olins, A. L. & Olins, D. E. Spheroid Chromatin Units (ν Bodies). Science 183, 330–332 (1974).). This observation lead the way to numerous discoveries around chromatin which ultimately culminated in the discovery of the 2.8 Angstrom high-resolution crystal structure 20 years ago in the year 1997 (Luger, K., Mäder, A. W., Richmond, R. K., Sargent, D. F. & Richmond, T. J. Crystal structure of the nucleosome core particle at 2.8 Å resolution. Nature 389, 251–260 (1997).)
References for this episodeAnchor-Based Bisulfite Sequencing (Ben Delatte)
Enhancer Communities in Adipocyte Differentiation (Susanne Mandrup)
Transposable Elements in Gene Regulation and Evolution (Marco Trizzino)
Hydroxymethylation Landscape in Immunecells (Marcela Sjöberg)
Single Cell Epigenomics in Neuronal Development (Tim Petros)
Oncohistones as Drivers of Pediatric Brain Tumors (Nada Jabado)
Characterization of Epigenetic States in the Oligodendrocyte Lineage (Gonçalo Castelo-Branco)
Multiple challenges of ATAC-Seq, Points to Consider (Yuan Xue)
The Effect of lncRNAs on Chromatin and Gene Regulation (John Rinn)
Epigenetic Clocks and Biomarkers of Ageing (Morgan Levine)
Epigenetic and Metabolic Regulation of Early Development (Jan Żylicz)
Multiple challenges of CUT&Tag (Cassidee McDonough, Kyle Tanguay)
The Role of Histone Dopaminylation and Serotinylation in Neuronal Plasticity (Ian Maze)
The Role of Blimp-1 in Immune-Cell Differentiation (Erna Magnúsdóttir)
Multimodal Characterization of Cellular Identity | CITE-Seq and Beyond (Peter Smibert, VP of 10X Genomics)
The Effect of Mechanotransduction on Chromatin Structure and Transcription in Stem Cells (Sara Wickström)
Single-Cell Technologies using Microfluidics (Ben Hindson, CSO of 10x Genomics)
Epigenome-based Precision Medicine (Eleni Tomazou)
Epigenetics and Epitranscriptomics in Cancer (Manel Esteller)
Enhancer-Promoter Interactions During Development (Yad Ghavi-Helm)
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